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Mouse PD-L1 / B7-H1 Protein, His Tag

分子别名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表达区间及表达系统(Source)

Mouse PD-L1, His Tag (PD1-M5220) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

Online(Phe 19 - Thr 238) NP_068693

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26。6 kDa。 The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation。

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0。22 μm filtered solution in PBS, pH7。4。 Normally trehalose is added as protectant before lyophilization。

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower。

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse PD-L1, His Tag (Cat. No. PD1-M5220) SDS-PAGE gel

Mouse PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Captured Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Chip can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 2.16 μM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Captured Mouse PD-L1, His Tag (Cat。 No。 PD1-M5220) on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag (Cat。 No。 PD1-M5259) with an affinity constant of 1。37 μM as determined in SPR assay (Biacore T200) (Routinely tested)。

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Mouse PD-1, Fc Tag (Cat。 No。 PD1-M5259) on ProteinA Biosensor, can bind Mouse PD-L1, His Tag (Cat。 No。 PD1-M5220) with an affinity constant of 1。5 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested)。

Protocol

 

文献引用(Citations)

  • Authors: Z Lin, et al

    Journal: Molecular Therapy: Oncolytics 43630

    Application: PD-L1 PD-1 Blockade assay (ELISA)

    Request for Full-text

  • Authors: Wang Aijun, et al.

    Journal: US20170355770A1 2017

    Application: Immunization

    Request for Full-text

背景(Background)

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

 

前沿进展

货号/价格

PD1-M5220-100ug / ¥3100

PD1-M5220-1mg / ¥18000

开发建议
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PD-L1靶点信息
英文全称:Programmed death-ligand 1
中文全称:程序性死亡配体1
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:50详情
最高研发阶段:批准上市
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