MABSol® Biotinylated Mouse PCSK9 (PC9-M82E1) is expressed from human HEK293 cells. It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1).
Predicted N-terminus: Gln 35
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag。 The protein has a calculated MW of 74。9 kDa。 The protein migrates as 20 kDa and 65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion。
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0。22 μm filtered solution in PBS, pH7。4。 Normally trehalose is added as protectant before lyophilization。
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Mouse PCSK9 on SDS-PAGE under reducing (R) condition。 The gel was stained overnight with Coomassie Blue。 The purity of the protein is greater than 95%。
Immobilized Mouse LDL R, His Tag (Cat. No. LDR-M52H8) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PCSK9, His Tag (Cat. No. PC9-M82E1) with a linear range of 2-16 ng/mL (QC tested).
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily。 Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum。 This protein plays a major regulatory role in cholesterol homeostasis。 PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation。 PCSK9 may also have a role in the differentiation of cortical neurons。 Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3)。