MABSol® Biotinylated Human PCSK9 (D374Y) (PCY-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7 (D374Y)).
Predicted N-terminus: Gln 31
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag。 The protein has a calculated MW of 73。7 kDa。 The protein migrates as 16 kDa,18 kDa and 65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion。
The D374Y mutation results in higher affinity of PCSK9 for LDLR.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Less than 1。0 EU per μg by the LAL method。
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower。
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition。 The gel was stained overnight with Coomassie Blue。 The purity of the protein is greater than 95%。
Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μl/well) can bind Biotinylated Human (D374Y) PCSK9 (Cat. No. PCY-H82E7 ) with a linear range of 0.02-0.5 μg/mL (QC tested).
Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).Especially,the D374Y mutation results in higher affinity of PCSK9 for LDLR.