Biotinylated Human CD52, His,Avitag (CD2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ser 36 (Accession # P31358-1).
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 13.5 kDa. The protein migrates as 16 kDa and 17-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE。
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human CD52, His,Avitag on SDS-PAGE under reducing (R) condition。 The gel was stained overnight with Coomassie Blue。 The purity of the protein is greater than 90%。
Immobilized Monoclonal Anti-Human CD52 Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD52, His,Avitag (Cat。 No。 CD2-H82E8) with a linear range of 0。078-1。25 μg/mL (QC tested)。
CAMPATH-1 antigen, also known as cluster of differentiation 52 (CD52), is a glycoprotein that in humans is encoded by the CD52 gene.It is widely expressed on the cell surface of immune cells, such as mature lymphocytes, natural killer cells (NK), eosinophils, neutrophils, monocytes/macrophages, and dendritic cells (DCs).ligation of cell surface CD52 molecules may offer costimulatory signals for T-cell activation and proliferation.