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Human 4-1BB Ligand / TNFSF9 (71-254) Protein, Fc Tag

分子别名(Synonym)

4-1BB Ligand,TNFSF9,CD137L

表达区间及表达系统(Source)

Human 4-1BB Ligand (71-254), Fc Tag (41L-H5265) is expressed from human 293 cells (HEK293)。 It contains AA Arg 71 - Glu 254 (Accession # P41273-1)。

Predicted N-terminus: Pro

蛋白结构(Molecular Characterization)

Online(Arg 71 - Glu 254) P41273-1

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 87.5 kDa. The protein migrates as 120-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions。

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower。

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human 4-1BB Ligand (71-254), Fc Tag (Cat. No. 41L-H5265) SDS-PAGE gel

Human 4-1BB Ligand (71-254), Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human 4-1BB Ligand (71-254), Fc Tag (Cat. No. 41L-H5265) at 2 μg/mL (100 μL/well) can bind Human 4‑1BB, His Tag (Cat. No. 41B-H5227) with a linear range of 0.2-1 ng/mL (QC tested).

Protocol

背景(Background)

Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9。 4-1BBL is the high affinity ligand of 4-1BB。 4-1BBL induces the proliferation of activated peripheral blood T-cells。 Also, 4-1BBL may have a role in activation-induced cell death (AICD)。 Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages。 As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases。

 

前沿进展

货号/价格

41L-H5265-100ug / ¥3500

41L-H5265-1mg (500ug X 2) / ¥23100

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