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Biotinylated Human Siglec-15 / CD33L3 Protein, Fc,Avitag™

分子别名(Synonym)

CD33 antigen-like 3,SIGLEC-15,CD33L3,sialic acid-binding Ig-like lectin 15,Siglec15,Siglec-15

表达区间及表达系统(Source)

Biotinylated Human Siglec-15, Fc,Avitag (SG5-H82F5) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).

Predicted N-terminus: Phe 20

蛋白结构(Molecular Characterization)

Online(Phe 20 - Thr 263) Q6ZMC9-1

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 54。8 kDa。 The protein migrates as 58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation。

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation。

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions。

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower。

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) SDS-PAGE gel

Biotinylated Human Siglec-15, Fc,Avitag on SDS-PAGE under reducing (R) condition。 The gel was stained overnight with Coomassie Blue。 The purity of the protein is greater than 95%。

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse Human chimeric MAb (5G12, Human IgG1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) with a linear range of 0.4-3 ng/mL (QC tested).

Protocol

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0.48-0.57 μg/mL (Routinely tested).

Protocol

 

背景(Background)

Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family。 Siglecs are cell surface proteins that bind sialic acid。 They are found primarily on the surface of immune cells and are a subset of the I-type lectins。 Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail。 Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation。 Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates。

 

前沿进展

货号/价格

SG5-H82F5-25ug / ¥3500

SG5-H82F5-200ug / ¥13800

开发建议
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联系电话:
400-682-2521(全国)
010-57737274(北京)
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Siglec-15靶点信息
英文全称:Sialic acid-binding Ig-like lectin 15
中文全称:唾液酸结合免疫球蛋白样凝集素-15
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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