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Cynomolgus Siglec-15 / CD33L3 Protein, Fc Tag

分子别名(Synonym)

CD33 antigen-like 3,SIGLEC-15,CD33L3,sialic acid-binding Ig-like lectin 15,Siglec15,Siglec-15

表达区间及表达系统(Source)

Cynomolgus Siglec-15, Fc Tag (SG5-C5253) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # A0A2K5UY47-1).

Predicted N-terminus: Phe 20

蛋白结构(Molecular Characterization)

Online(Phe 20 - Thr 263) A0A2K5UY47-1

This protein carries a human IgG1 Fc tag at the C-terminus。

The protein has a calculated MW of 52.7 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cynomolgus Siglec-15, Fc Tag (Cat. No. SG5-C5253) SDS-PAGE gel

Cynomolgus Siglec-15, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus Siglec-15, Fc Tag (Cat。 No。 SG5-C5253) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0。4-6 ng/mL (QC tested)。

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Cynomolgus Siglec-15, Fc Tag (Cat。 No。 SG5-C5253) on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0。62 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)。

Protocol

 

背景(Background)

Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

 

前沿进展

货号/价格

SG5-C5253-100ug / ¥3500

SG5-C5253-1mg (500ug X 2) / ¥23100

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Siglec-15靶点信息
英文全称:Sialic acid-binding Ig-like lectin 15
中文全称:唾液酸结合免疫球蛋白样凝集素-15
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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