Human Glypican 2, Fc Tag (GP2-H5256) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gly 554 (Accession # Q8N158-1).
Predicted N-terminus: Ser 24
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 84.5 kDa.
Lyophilized from 0。22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7。5。 Normally trehalose is added as protectant before lyophilization。
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system。 Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons。 Cerebroglycan has especially high affinity for laminin-1。 GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis。 Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models。 A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation。