Cynomolgus / Rhesus macaque PD-L1, His Tag (PD1-C52H4) is expressed from human 293 cells (HEK293)。 It contains AA Phe 19 - Arg 238 (Accession # G7PSE7)。 In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus。
Predicted N-terminus: Phe 19
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.1 kDa. The protein migrates as 37-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus / Rhesus macaque PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) at 1 μg/mL (100 μL/well) can bind MPDL3280A with a linear range of 0.4-3 ng/mL (QC tested).
Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface, can bind Cynomolgus Rhesus / macaque PD-L1, His Tag (Cat。 No。 PD1-C52H4) with an affinity constant of 23。3 nM as determined in a SPR assay (Biacore T200) (Routinely tested)。
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.