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Siglec-15蛋白 - Cell based assay验证生物活性

背景
Siglec-15是唾液酸结合Ig样凝集素(Siglecs)家族的一员,在M2型巨噬细胞、髓系细胞(MDSC)、树突状细胞、B细胞与破骨细胞中表达。相关研究表明,对于绝大多数PD-L1阴性肿瘤患者中,Siglec-15很可能是主要免疫抑制因子,该蛋白与PD-L1是互斥表达的,即PD-L1和Siglec-15在肿瘤细胞内的表达是此消彼长的。Siglec-15可以持续抑制T细胞的活性,并且表现出了满足正常化癌症免疫治疗的主要特征,对于那些PD-1/PD-L1治疗无效的癌症患者,Siglec-15可能为他们带来新的免疫治疗方案。
高性能的Siglec-15蛋白更能助力于药物的研发,ACROBiosystems开发的Siglec-15蛋白活性经Cell based assay /BLI /SPR /ELISA多种技术验证,并且免费提供相应的protocol,更能帮助您缩短研发周期。
产品特点
Cell based assay/ BLI/ SPR/ ELISA多种技术验证Siglec-15蛋白活性,并免费提供Protocol

Cell based assay

Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0.48-0.57 μg/mL.

Protocol

BLI

Loaded Anti-Siglec-15 MAb (Mouse IgG) on AMC Biosensor, can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 59.7 pM as determined in BLI assay (ForteBio Octet Red96e).

Protocol

SPR

Immobilized Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4.49 μM as determined in a SPR assay (Biacore T200).

Protocol

ELISA

Immobilized Mouse Human chimeric MAb (5G12, Human IgG1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) with a linear range of 0.4-3 ng/mL.

Protocol

多种属(Human, Mouse, Cyno)& 多标签(Fc, His, Avi tag)
Cat. No. Species Product Description
SG5-H52H3 Human Siglec-15 / CD33L3 Protein, His Tag
SG5-H5253 Human Siglec-15 / CD33L3 Protein, Fc Tag
SG5-H82F5 Human Biotinylated Siglec-15 / CD33L3 Protein, Fc, Avitag™
SG5-H82E9 Human Biotinylated Siglec-15 / CD33L3 Protein, His, Avitag™ (recommended for biopanning)
SG5-M52H7 Mouse Siglec-15 / CD33L3 Protein, His Tag
SG5-C52H6 Cynomolgus Siglec-15 / CD33L3 Protein, His Tag
SG5-C5253 Cynomolgus Siglec-15 / CD33L3 Protein, His Tag

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