搜索
>产品 > 荧光标记蛋白

荧光标记蛋白

近年来,流式细胞术被广泛应用于生物药研发和生产的各个环节。荧光标记蛋白作为流式检测的首选试剂,因其预先标记了荧光素基团,可通过一步染色完成检测,既简化了检测流程、节省了检测时间,又避免了因使用二抗而产生的非特异背景。然而,高质量荧光标记蛋白的生产面临着诸多难题,如标记后蛋白活性难以维持、产品的批间一致性难以控制等,目前市场上少有高质量荧光标记蛋白可供购买。

ACROBiosystems作为专注于生物药研发领域的蛋白供应商,利用专业的蛋白研发及标记平台、稳定株开发平台和流式细胞分析平台,开发了一系列包括FITC标记和PE标记的靶点蛋白,适用于基于流式细胞术的分析试验,如CAR阳性表达率的检测和流式细胞术筛选抗体候选药物等。

产品特色
  1. 标记后蛋白活性经流式细胞术验证
  2. 可提供配套流式检测protocol供参考
  3. 标记前后活性保持一致
  4. 高批间一致性
应用案例
流式细胞术检测Anti-CD19 CAR阳性表达率
FACS Analysis of Anti-CD19 CAR Expression
293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251, 10 μg/ml) and C. FITC-labeled protein control. A. Nin-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251) (QC tested).
Protocol
基于流式细胞术的配体结合试验
Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1μl stock solution per million cells (QC tested).
Protocol
基于流式细胞术的中和抗体筛选试验
FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat.No.PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing antihuman PD-L1 antibody. The IC50 is 0.29 μg/ml (QC tested).
Protocol
质控
高批间一致性
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows very high batch-to-batch consistency.
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above ELISA analysis against Anti-MSLN Antibody. The result shows very high batch-to-batch consistency.
标记前后活性保持一致
Binding activity of the Human MSLN, Fc Tag before and after FITC labeling was evaluated in the above ELISA analysis. The result shows that FITC-Labeled and Unconjugated Human MSLN, Fc Tag have almost the same level of binding activity.
稳定性好
Binding activity of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows that the FITC-labeled Human MSLN is stable at 37℃ for 24 hours without performance reduction.
Binding activity of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows that the FITC-labeled Human MSLN is stable within three freeze-thaw cycles without performance reduction.
产品列表
PE Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
SIRP alpha SIA-HP252 Human PE-Labeled Human SIRP alpha, Fc Tag
(recommended for neutralizing assay)
HEK293
PD-1 PD1-HP2F2 Human PE-Labeled Human PD-1, Fc Tag, His Tag
(recommended for neutralizing assay)
HEK293
Mesothelin MSN-HP223 Human PE-Labeled Human Mesothelin (296-580) HEK293
FITC Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
Mesothelin MSN-HF25x Human FITC-Labeled Human Mesothelin (296-580), Fc Tag HEK293
MSN-HF223 Human PE-Labeled Human Mesothelin (296-580) HEK293
MSN-HF253 Human FITC-Labeled Human Mesothelin (296-580), Fc Tag HEK293
CD30 CD0-HF2H3 Human FITC-Labeled Human CD30, His Tag HEK293
SLAMF7 SL7-HF2H7 Human FITC-Labeled Human SLAMF7, His Tag HEK293
BCMA BCA-HF2H1 Human FITC-Labeled Human BCMA, His Tag HEK293
BCA-HF254 Human FITC-Labeled Human BCMA, Fc Tag HEK293
Siglec-2 SI2-HF2H6 Human FITC-Labeled Human Siglec-2 HEK293
CD19 CD9-HF251 Human FITC-Labeled Human CD19 (20-291), Fc Tag HEK293
CD9-HF2H2 Human FITC-Labeled Human CD19 (20-291) HEK293
Glypican 3 GP3-HF258 Human FITC-Labeled Human Glypican 3, Fc Tag HEK293
GP3-HF2H1 Human FITC-Labeled Human Glypican 3, His Tag HEK293
Protein L RPL-PF141 - FITC-Labeled Recombinant Protein L E.coli
BAFF BAF-HF268 Human FITC-Labeled Human BAFF / TNFSF13B / CD257 Protein, Fc Tag HEK293
产品定制

为满足生物药研发和生产领域客户的需求,ACROBiosystems正在积极开发高质量的荧光标记蛋白产品。如果您愿意分享您在这个领域的想法和需求,我们将非常感谢。如果您有任何建议或定制要求,请随时与我们联系。

联系我们

消息提示

请输入您的联系方式,再点击提交!

确定