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全长CD20蛋白

免费提供经ELISA/ SPR验证的Protocol

背景
CD20是淋巴癌、白血病和某些自身免疫疾病等治疗的热门目标靶点,其在免疫治疗领域的应用非常广泛。但因为全长CD20蛋白的表达难度高、活性不稳定等因素,目前市面上很难找到合适的CD20蛋白用于活性分析。
为了解决这些问题,ACROBiosystems专门设计开发了全球独有的分别由HEK293 cells和Insect cells表达的多次跨膜全长CD20蛋白,并对蛋白的纯度、活性等性能进行了多方位的验证,推荐用于ELISA/ SPR/ BLI等实验。除此之外,ACROBiosystems还可提供用于免疫、CD20-CAR阳性率检测的全长CD20蛋白,欢迎咨询。
应用
产品列表
Molecule Cat. No. Host Product Description
CD20/ MS4A1 CD0-H52H3 HEK293 Human CD20/ MS4A1 Full Length Protein, His Tag
CD0-H55Ha Insect cells-HI5 Human CD20/ MS4A1 Full Length Protein, His Tag
产品特点
全长CD20蛋白由真核系统表达具有完整的天然构象(同时具有small loop和big loop)
全长CD20蛋白能提供更好的活性

A) HEK293 Cell (Full Length)

Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with a linear range of 2-16 ng/mL.

Protocol

B) Insect Cell (Full Length)

Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with a linear range of 1-16 ng/mL.

Protocol

C) E.coli (Extracellular)

Immobilized Rituximab at 5 μg/mL (100 μL/well) can bind Human CD20, TrxA Tag, His Tag (E.coli) with a linear range of 78-2500 ng/mL.

ACRO提供的全长CD20蛋白活性经ELISA/ SPR验证,可免费提供相应的Protocol
ELISA验证, 全长CD20蛋白相关产品: Cat. No. CD0-H52H3 (HEK293表达) & Cat. No. CD0-H55Ha (Insect cells表达)

Immobilized Ofatumumab at 10 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with a linear range of 2-31 ng/mL.

Protocol

Immobilized Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) at 2 μg/mL (100 μL/well) on Nickel Coated Plate, can bind rituximab with a linear range of 0.313-1.25 μg/mL.

Protocol

Immobilized Ofatumumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with a linear range of 2-31 ng/mL.

Protocol


SPR验证, 全长CD20蛋白相关产品: Cat. No. CD0-H52H3 (HEK293表达) & Cat. No. CD0-H55Ha (Insect cells表达)

MabThera® (Rituximab) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with an affinity constant of 1.77 nM as determined in an SPR assay (Biacore T200).

Protocol

Ofatumumab (Human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with an affinity constant of 3.57 nM as determined in an SPR assay (Biacore T200).

Protocol

MabThera® (Rituximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with an affinity constant of 1.21 nM as determined in an SPR assay (in the presence of DDM) (Biacore T200).

Protocol

Ofatumumab (Human IgG1) captured on CM5 chip via ant-human IgG Fc antibodies surface can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with an affinity constant of 2.977 nM as determined in an SPR assay (Biacore T200).

Protocol

*ACRO还可提供用于免疫/ CD20-CAR阳性率检测的全长CD20蛋白 联系我们
*如果您希望ACRO补充更多该产品的实验设计和应用数据,请 点击咨询

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